Reply To: worth to use IGF 1?

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#1565
StoneMade
Participant

hey there guys once again. I’ve been thinking about my previous post and I realized that it is not fully made. I mean, I thought that there are some other things that misses that post so I decided to update it (plus, as I have mentioned in my first post, if I would have something else to update – I would, so here I’m doing it).

one of the most important thing that got out of my mind is reconstitution. that’s essential. in order to tell you more about this I’ve got to say that there are many other threads and articles that IMO are written way much better than I ever could do it, so I suggest you to find those great threads on reconstitution with AA (which stands for acetic acid), you people got to check it out there (because, in addition, as I said, my thread is by far not enough, as I said, it is only basic stuff, if you’re seriously intending to use it, you’ve got to research a lot firstly!). but, just about always you should not have to be worried about reconstituting it yourself. this means that nearly all of the manufacturers nowadays (but maybe already all of them since there are controls) are suspending their LR 3 in either BA or AA for you, so that’s very good.

one other thing which is also important and many people is the storage. well, look, you’ve gotta know that the stability of a liquid solution of the LR 3 IGF 1 has been monitored for a period of 2 years at a storage conditions of -20 C, +4 C, +22 C, and lastly +37 C. well, the final concentration of the LR 3 IGF 1 has been in acetic acid (AA). at some various time points (I guess it is not so important to mentioned them here), those samples have been taken and were compared to a lyophilized control (which is stored at + 4 C). what I do think that might be important for you people to know is the list that I have made below, those are the stability results for each respective storage condition which I hope is going to help you to properly store your doses.

at the storage condition of -20 C (which is -4 F) the biological potency has had absolutely no change up to 2 years, exactly the same goes to the immunological activity which has had absolutely no change up to 2 years. the same goes to the following 2: mobility of protein no change up to 2 years as well as elution profile by reversed phased HPLC no change up to 2 years.

at the storage condition of +4 C (which is 39.2 F) everything went exactly the same as with -20 C.
Biological Potency No Change up to 2 years. Immunological Activity No Change up to 2 years. Mobility of Protein No Change up to 2 years. Elution Profile by reversed phased HPLC No Change up to 2 years; exactly as with the storage condition of +22 C (which is 71.6 F) with no changes…. Biological Potency No Change up to 2 years. Immunological Activity No Change up to 2 years. Mobility of Protein No Change up to 2 years. Elution Profile by reversed phased HPLC No Change up to 2 years.
the only one that did have had a change has been at the storage condition of +37 C (which is 98.6 F) because everything goes exactly the same with the exception that it did have had changes up to 2 years. however at this temperature it still didn’t had any change up to 1 year as it goes like the following: Biological Potency No Change up to 1 year. Immunological Activity No Change up to 1 year. Mobility of Protein No Change up to 1 year. Elution Profile by reversed phased HPLC No Change up to 1 year.
that’s pretty much it but I also wanted to add a conclusion which I haven’t done on my first thread but I do know that many people want it (when I was searching for such information, I was always searching for conclusions as I thought that they were really helpful) and so, here’s the conclusion to both posts I wrote here:
there is no big or significant difference in the potency of the LR 3 IGF 1 that is associated with the storage of the liquid formulation when it is being stored at this range of temperatures. there is absolutely no evidence for loss of the biological activity at any of the tested temperatures that I have given you above when it is stored as a liquid product. having that said, you can store it nearly however you want, except for not letting it at temperatures more than like +30 C for more than a year. but generally, as much as you can see it yourself, IGF can be pretty stable for even a year and more at a room temperature. but then again, if you do want or plan to keep it around for more than a year then I would recommend you to stick it into the fridge. and now, since I do know that there are situations when we keep it around for more than a year, IMO, the best way to store the LR 3 that is suspended in BA is in the freezer. the BA is not going to allow it to freeze. and also, if you are having it suspended in AA then I would say that you’ve got to store it in the fridge.

again, hoping this is going to be helpful to you guys, at least I really tried to help with whatever I could.